MAP Kinase p38 alpha (MAPK14, Mitogen-activated Protein Kinase p38 alpha, Mitogen-activated Protein Kinase 14, CSBP, Cytokine Suppressive Anti-inflammatory Drug-binding Protein, CASID-binding Protein, SAPK2A, CSBP, CSBP1, CSBP2, C
Artikelnummer:
USB-M2352-01
Hersteller Artikelnummer:
M2352-01
Alternativnummer:
USB-M2352-01-100
Hersteller:
US Biological
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
ELISA, FC, WB
Immunogen:
MBP-p38 MAP kinase recombinant protein.
p38 MAP kinase (MAPK) is also called RK (3) or CSBP (2). It is the mammalian homologue of the yeast HOG kinase. It participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP (kinase, alpha, beta, gamma and delta) have been identified. Like the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3 and SEK activate p38 MAP kinase by phosphorylation at threonine 180 and tyrosine 182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8). Applications: Suitable for use in ELISA, Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1:2000. Incubate membrane with diluted M2352-01 in 1X TBS, 5% nonfat dry milk, 0.1% Tween-20 at 4C with gentle shaking, overnight. Flow Cytometry: 1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
