Human b1 integrins purified from Triton X-100 lysates of human lung, liver, and skeletal muscle tissues. Specifically selected due to its increased reactivity with T-lymphoblasts upon Mn2+ stimulation.
The beta1 or VLA integrin subgroup contains at least 10 members, each with a distinct alpha subunit non-convalently associated with the common b1 subunit. Functional activation of integrins can be experimentally induced by addition of Mg2+ or Mn2+, or by using one of several stimulatory antibodies that are available. Intracellular signals generated as a result of cell activation also modulate the confirmation and affinity of integrins in cellular systems. Antibodies which have the ability to discriminate between different states of integrin activation are exceptionally useful for studies on the regulation of activation. This antibody recognizes epitopes in the 355-425 region of the common b1 subunit of VLA integrins, whose expression parallels the ligand binding activity of these adhesion receptors induced by various extracellular and intracellular stimuli. Applications: Suitable for use in ELISA, Western Blot, FACS and Immunohistochemistry. Other applications not tested. Recommended Dilutions: FACS: 2.5ug/5x10e5 cells Western Blot : non-reducing conditions only Immunohistochemistry: Fresh frozen, or light fixation, with 4% PFA permeabilized with 0.1% triton X-100 in the presence of Mn2+ as the epitope of HUTS-4 is dependent upon Mn2+ ion. Note antibody only stains activated beta1 integrins. Activated beta-1 integrins are typically found in wound regions for instance Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
