Integrin beta1, Activating (HRP), IgG1, Clone: [2Q849 P4G11], Mouse, Monoclonal
Artikelnummer:
USB-I7661-38-HRP
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| Artikelname: | Integrin beta1, Activating (HRP), IgG1, Clone: [2Q849 P4G11], Mouse, Monoclonal |
| Artikelnummer: | USB-I7661-38-HRP |
| Hersteller Artikelnummer: | I7661-38-HRP |
| Alternativnummer: | USB-I7661-38-HRP-100 |
| Hersteller: | US Biological |
| Wirt: | Mouse |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IHC, IP |
| Immunogen: | Human IL-1b activated endothelial cells. |
| Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry, Immunoprecipitation and ELISA. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 2ug/ml (use on numerous cell lines as long as Ca2+ is present), Immunoprecipitation: 5ug/400ul lysate (0.5% triton PBS plus Ca++) FACS: 2-5ug/10e6 cells, in the presence of Ca++. Stimulation of cells: 2-10ug/ml per 10e6 cells. Binding buffer must contain 1mM Ca++ Immunohistochemistry: For use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and paraffin embedded (1:500, citrate HEIR treated, Qin et al 2003). Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers. Optimal dilution determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody. |
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