Recombinant corresponding to aa19-1141 from mouse Integrin a11 expressed in CHO cells (P61622). Species sequence homology: Recombinant human (Integrin a11 (2%), Integrin a2 and Integrin a10 (5% ).
Integrin a11 ( ITGA11) is a 155-160kD member of the integrin alpha chain family of molecules. It preferentially forms a cell surface heterodimer with b1 Integrin. In particular, it is further classified as a collagen-binding group member, showing a preference for binding to collagen I and II, plus group A Streptococcal Scl1 protein. ITGA11 is expressed by embryonic mesenchymal cells in areas that incorporate collagen during development. In adult tissue, fibroblasts express ITGA11, and a collagen:ITGA11 interaction likely contributes to the formation of myofibroblasts. Mature mouse ITGA11 is an 1166aa type I transmembrane glycoprotein. It contains a large 1119aa extracellular domain (ECD) aa23-1141 plus a short 24aa cytoplasmic region. The ECD shows 7x60aa FG-Gap repeats that generate b-propellers aa24-650 with an intervening I domain that binds collagen aa164-345. There is one potential splice variant that shows an eight aa substitution for aa89-119 coupled to a seven aa insertion after Gly690. Over aa23-1141, mouse ITGA11 shares 95-90aa identity with rat and human ITGA11, respectively. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunocytochemistry: 5-15ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
