Applications: Suitable for use in the inhibition of attachment of HT29 cells to vitronectin, Inhibition of attachment of carcinoma and melanoma cells to vitronectin, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, indirect Flow Cytometry and ELISA. Other applications not tested. Recommended Dilutions: Inhibition of attachment of HT29 cells to vitronectin: >1:1000 Immunocytochemistry (acetone fixed only): 1:1500 For use on human carcinomas, normal epithelium and keratinocytes. Immunohistochemistry: Acetone or paraformaldehyde fixed tissues. Not recommended for paraffin sections. Optimal dilution determined by the researcher. Control: Epithelium, keratinocytes Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.