Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix (ECM). Integrins have diverse roles in several biological processes including cell migration during development and wound healing, cell differentiation, and apoptosis. Their activities can also regulate the metastatic and invasive potential of tumor cells. They exist as heterodimers consisting of alpha and beta subunits. Some alpha and beta subunits exhibit specificity for one another and may be designated as a VLA (very late antigen) member. Heterodimers often preferentially bind certain cell adhesion molecules, or constituents of the ECM. Although they have no catalytic activity, integrins can be part of multimolecular signaling complexes known as focal adhesions. Applications: Suitable for use in the inhibition of attachment of HT29 cells to vitronectin, Inhibition of attachment of carcinoma and melanoma cells to vitronectin, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, Flow Cytometry and ELISA. Other applications not tested. Recommended Dilutions: Inhibition of attachment of HT29 cells to vitronectin: > 1:1000 Immunocytochemistry (acetone fixed only): 1:1500 (human carcinomas, normal epithelium and keratinocytes) Immunohistochemistry: Acetone or paraformaldehyde fixed tissues. Optimal dilution to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
