Recombinant protein corresponding to Val89-Leu169 from human Integrin a9, expressed in E. coli.
Integrin alpha 9 is a 150kD type I transmembrane glycoprotein that is widely expressed and is found on smooth muscle, keratinocytes, skeletal muscle and hepatocytes. It principally associates with the beta 1 Integrin. alpha 9 beta 1 binds to VEGF-C and D, and osteopontin. The 951 amino acid (aa) extracellular domain of mouse Integrin alpha 9 contains three beta -propellor repeats and multiple PheGly-GlyAlaPro repeats. A potential proteolytic cleavage site in human alpha 9 ECD is absolutely conserved in mouse alpha 9 ECD (Arg 566-Val 567). The ECD of mouse Integrin alpha 9 shares 95% and 89% aa sequence identity to rat and human Integrin alpha 9, respectively. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml using Tera2 human embryonic lung carcinoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
