Recombinant protein corresponding to Try31-Val979 from mouse Integin alpha9 expressed in NSO cells (NP_598482).
Integrin alpha9 is a 150kD type I transmembrane glycoprotein that is widely expressed and is found on smooth muscle, keratinocytes, skeletal muscle and hepatocytes. It principally associates with the beta1 integrin. Alpha9beta1 binds to VEGF-C and D, and osteopontin. The 951 amino acid (aa) extracellular domain of mouse integrin alpha9 contains three beta-propellor repeats and multiple PheGly-GlyAlaPro repeats. A potential proteolytic cleavage site in human alpha9 ECD is absolutely conserved in mouse alpha9 ECD (Arg 566-Val 567). The ECD of mouse integrin alpha9 shares 95% and 89% aa sequence identity to rat and human integrin alpha9, respectively. Applications: Suitable for use in Immunohistochemistry, Western Blot, Flow Cytometry and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml. Tested on recombinant mouse Integrin alpha9. Flow Cytometry: 2.5ug labels 10e6 cells. Tested on D3 mouse embryonic stem cells. Immunohistochemistry: 1-15ug/ml in perfusion fixed frozen sections of mouse liver tissue Optimal dilution determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
