Recombinant protein corresponding to Phe38-Phe1007 from mouse Integrin alpha8, expressed in CHO cells.
Integrin alpha8 is a 170-200kD member of the integrin family of adhesion molecules. It forms an exclusive noncovalent heterodimer with integrin beta-1. Alpha-8/beta-1 romotes both cell adhesion and survival and is known to bind to fibronectin, the latency-associated peptide in latent TGF-beta 1 and nephronectin. Mouse integrin alpha-8 is a 1025aa type I transmembrane glycoprotein. It contains a 969aa extracellular region (aa38-1006) and a 29aa cytoplasmic domain. One isoform may exist that shows a truncation after Ala675 of the precursor. In the ECD, mouse Integrin alpha8 shares 96% and 90% aa sequence identity with rat and human Integrin alpha-8 protein, respectively. Applications: Suitable for use in Direct ELISA, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.25ug/ml using mouse lung tissue Immunocytochemistry: 5-15ug/ml using immersion fixed 4T1 mouse breast cancer cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
