Hepatitis C full length nucleocapsid recombinant proretn (truncated p22) produced in Yeast expression system. Amino Acid Sequence: 1 mstnpkpqrk tkrntnrrpq dvkfpgggqi vggvyllprr gprlgvratr ktsersqprg 61 rrqpipkarr pegrtwaqpg ypwplygneg cgwagwllsp rgsrpswgpt dprrrsrnlg 121 kvidtltcgf adlmgyiplv gaplggaara lahgvrvled gvnyatgnlp gcsfsiflla 181 llscltvpas a Applications: Suitable for use in ELISA, Western Blot, solid phase antigen-antibody titration assay and T-Cell Activation. Other applications not tested. Recommended Dilutions: solid phase antigen-antibody titration assay: Dilute the antigen preparation 10 to 20-fold in PBS. Western Blot: 10ng range depending on the nature and affinity of the secondary detection reagent. ELISA: HCV converted human serum polyclonal antibodies yield titers of 1:10, 000 or greater at 100ng of immobilized protein under standard ELISA conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molekulargewicht:
18
Reinheit:
~90% by SDS-PAGE, reduced. Purified by immunoaffinity chromatography methods.
Formulierung:
Supplied as a liquid in 50mM Tris, pH 8.0, 0.1mM sodium chloride. No stabilizers or preservatives added.
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