Human Chorionic Gonadotropin (hCG) is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. hCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the a-and b-subunits. The a-subunit of hCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP), and hCG but negative for EMA and AFP is probably a choriocarcinoma. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilution to be determined by the researcher. Recommended Pair: Suitable for use with C5069-12 in a sandwich ELISA. Affinity Constant: 4x10e10L/mole Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
