N-Acetyl Alpha-D-Glucosaminidase (NAGLU) Recombinant, Rat
Artikelnummer:
USB-156015
Hersteller Artikelnummer:
156015
Alternativnummer:
USB-156015-10,USB-156015-50,USB-156015-200
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Source: Recombinant Rat from E. coli Accession No: D3ZZL3 Fragment: Asn449~Asn709 (Accession No: D3ZZL3) Sequence: MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-NE VVYALMAELG WRKDPVPDLV AWVSSFASRR YGVSQPDAVA AWRLLLRSVY NCSGEACSGH NRSPLVKRPS LQMSTAVWYN RSDVFEAWRL LLRAAPNLTA SPAFRYDLLD VTRQAVQELV SSCYEEARTA FLNQDLDLLL RAGGLLTYKL LPSLDELLAS NSHFLLGTWL DQAREVAVSE SEAQFYEQNS RYQITLWGPE GNILDYANKQ LAGLVADYYQ PRWCLFLGTL AHSLARGIPF QQHQFEKSVF PLEQAFINN Epitope Tag: N-terminal Tags: His-tag and GST-tag Molecular Weight: 56.6kD Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.
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