Recombinant protein corresponding to aa5075-5179 with an N-Terminal His Tag from human Mucin 2 expressed in E. coli. Swiss/UniProt Accession: Q02817. Amino Acid Sequence: CSTVPV TTEVSYAGCT KTVLMNHCSG SCGTFVMYSA KAQALDHSCS CCKEEKTSQR EVVLSCPNGG SLTHTYTHIE SCQCQDTVCG LPTGTSRRAR RSPRHLGSG Predicted Isoelectric Point: 8.1 Predicted Molecular Weight: 12.7kD Accurate Molecular Weight: 15kD as determined by SDS-PAGE reducing conditions Subcellular Location: Secreted Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
