Recombinant protein corresponding to aa410-525 with N-Terminal His Tag from mouse MUC1, expressed in E. coli. Uniprot Number: Q02496 Amino Acid Sequence: S PQLSVGVSFF FLSFYIQNHP FNSSLEDPSS NYYQELKRNI SGLFLQIFNG DFLGISSIKF RSGSVVVEST VVFREGTFSA SDVKSQLIQH KKEADDYNLT ISEVKVNEMQ FPPSA Predicted Isoelectric Point: 6.0 Predicted Molecular Weight: 14.6kD Accurate Molecular Mass: 10kD as determined by SDS-PAGE reducing conditions Phenomenon Explanation: The possible reasons that the actual band size differs from the predicted are as follows: 1.Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. Subcellular Location: Membrane, Nucleus, Chromosome Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C.. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride pH 8.0, 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, 0.05% Proclin 300. Reconstitute with sterile 20mM Tris, 150mM sodium chloride pH 8.0 to a concentration of 0.1-1mg/ml. Do not vor
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