Mindbomb homolog 1 (MIB1) is an E3 ligase that facilitates the ubiquitination and the subsequent endocytosis of the Notch ligands, Delta and Jagged (1,2). MIB1 appears to promote the ubiquitination and degradation of death-associated protein kinase (DAPK1) in vitro (3). Expression of MIB1 is seen in both adult and embryonic murine tissues (4). Recently, MIB1 was reported to regulate the extrinsic cell death pathway by binding to cellular FLICE-like inhibitory proteins (cFLIP-L and cFLIP-S), which reduces the interaction of caspase-8 with cFLIP and leads to cell death (5). MIB1 is also involved in T and marginal zone B (MZB) cell development in the lymphopoietic niches (6). Source: Recombinant Mouse from E. coli Accession No: Q8R516 Fragment: Val546~Asn784 (Accession No: Q8R516) Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-VEVLT EVPGIDVTAT NSQGFTLLHH ASLKGHVLAV RKILARARQL VDAKKEDGFT ALHLAALNNH REVAQVLIRE GRCDVNVRNR KLQSPLHLAV QQAHLGLVPL LVDAGCSVNT EDEEGDTALH VALQRHQLLP LVADRAGGDP GPLQLLSRLQ ASGLPGCTEL TVGAAVACFL ALEGADVSYA NHRGRSPLDL ATEGRVLKAL QGCAQRFRER QAGGGGGVPP GPRHVLSTPN TVTN Epitope Tag: N-terminal Tags: His-tag and T7-tag Molecular Weight: 29.1kD Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.
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