Recombinant protein corresponding to aa225-390 with an N-Terminal His-Tag from mouse MMP9 expressed in E. coli Swiss/UniProt Accession: P41245. Predicted Isoelectric Point: 6.2 Predicted Molecular Weight: 19.6kD Accurate Molecular Mass: 20kD as determined by SDS-PAGE reducing conditions. Subcellular Location: Secreted, extracellular matrix Amino Acid Sequence: SNGAPC HFPFTFEGRS YSACTTDGRN DGTPWCSTTA DYDKDGKFGF CPSERLYTEH GNGEGKPCVF PFIFEGRSYS ACTTKGRSDG YRWCATTANY DQDKLYGFCP TRVDATVVGG NSAGELCVFP FVFLGKQYSS CTSDGRRDGR LWCATTSNFD TDKKWGFCPD Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -70C. Stable for 6 months after receipt. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH8.0. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 5% Proclin300. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH8.0 to a concentration of 0.1-1mg/ml. Do not vortex
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