Recombinant protein corresponding to aa210-403 with an N-Terminal His Tag from mouse AFM, expressed in E. coli. Swiss/Uniprot: O89020. Amino Acid Sequence: T QYLKASSSYQ RNVCGALIKF GPKVLNSINV AVFSKKFPKI GFKDLTTLLE DVSSMYEGCC EGDVVHCIRS QSQVVNHICS KQDSISSKIK VCCEKKTLER EACIINANKD DRPEGLSLRE AKFTESENVC QERDSDPDKF FAEFIYEYSR RHPDLSTPEL LRITKVYMDF LEDCCSRENP AGCYRHVEDK FNE Predicted Isoelectric Point: 6.2 Predicted Molecular Weight: 23.5kD Accurate Molecular Weight: 23kD as determined by SDS-PAGE reducing conditions Subcellular Location: Secreted Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Supplied as lyophilized powder from PBS, pH 7.4, 0.01% Tween-80, 5% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-0.5mg/ml. Do not vortex.
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