Recombinant protein corresponding to aa17-243 with two N-terminal Tags, His-Tag and GST-Tag from porcine ADP, expressed in E. coli (Q6PP07) Amino Acid Sequence: MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-GQET TEKPGALLPM PKGACAGWMA GIPGHPGHNG TPGRDGRDGV PGEKGEKGDT GLTGPKGDTG ESGVTGVEGP RGFPGIPGRK GEPGESAYVY RSAFSVGLET RVTVPNMPIR FTKIFYNQQN HYDVTTGKFH CNIPGLYYFS FHITVYLKDV KVSLYKKDKA VLFTYDQYQD KNVDQASGSV LLYLEKGDQV WLQAYGDEEN NGVYADNVND SIFTGFLLYH NIE Predicted Molecular Weight: 56.7kD Predicted Isoelectric Point: 6.0 Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Included Protein Molecular Weight Marker: 168631: Protein Molecular Weight Marker, Unstained Ready to use, No heating, diluting or reducing agents needed. MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD Double intensity: 10kD, 18kD, 26kD Supplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels, 7ul/well for large gels. Store at -20C Stable for 6 months after receipt. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
