Source: Recombinant Rat from E. coli Accession No: P29293 Fragment: Ile43~Gly291 (Accession No: P29293) Sequence: MGHHHHHHSG SEF- IVGGQTSS PGAWPWMVSL QIFTSHNSRR YHACGGSLLN SHWVLTAAHC FDNKKKVYDW RLVFGAHEIE YGRNKPVKEP QQERYVQKIV IHEKYNAVTE GNDIALLKVT PPVTCGDFVG PGCLPHFKSG PPRIPHTCYV TGWGYIKDNA PRPSPVLMEA RVDLIDLDLC NSTQWYNGRV TSTNVCAGYP EGKIDTCQGD SGGPLMCRDS VDSPFVIVGI TSWGVGCARA KRPGVYTATW DYLDWIASKI G Epitope Tag: N-terminal His-Tag Molecular Weight: 29.1kD Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.
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