Sandwich ELISA quantitative detection kit for Human Chitinase 3-like 1 /YKL-40 in cell culture supernates, serum, plasma (heparin, EDTA) and urine. Chitinase-3-like protein 1, also called CHI3L1 or YKL-40 is a secreted glycoprotein that is approximately 40kD in size that in humans is encoded by the CHI3L1 gene. This gene is mapped to 1q32.1. Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. Assay Range: 62.5pg/ml-4000pg/ml Sensitivity: < 10 pg/ml Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: P36222 Entrez Gene ID: 1116 Kit Components: 96-well plate pre-coated with anti-Chitinase 3-like 1 /YKL-40, Human Lyophilized recombinant Chitinase 3-like 1 /YKL-40, Human standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml Storage and Handling: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles.
