Sandwich ELISA quantitative detection kit for Rat CD86/ B7-2 in cell culture supernates and serum. Cluster of Differentiation 86 (also known as CD86 and B7-2) is aprotein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma. Assay Range: 62.5pg/ml-4000pg/ml Sensitivity: < 10 pg/ml Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: Q62810 Entrez Gene ID: 56822 Kit Components: 96-well plate pre-coated with anti-CD86/B7-2, Rat Lyophilized recombinant CD86/B7-2, Rat standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml Storage and Handling: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles.
