CD163 (Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich (SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangiers disease, reumatoid arthritis etc. Uniprot ID: Q86VB7 Entrez Gene ID: 9332 Intended Use: CD163 BioAssay(TM) ELISA Kit is a sandwich ELISA for quantitative detection of human CD163 in cell culture supernates, serum and plasma (heparin). Range: 1.56-100ng/ml Sensitivity: <150 pg/ml Specificity: Natural and recombinant human CD163 Crossreactivity: No detectable crossreactivity with other relevant proteins Intra-Assay CV(%): 4.2, 4.5, 5.3 Inter-Assay CV(%): 6.7, 5.4, 5.6 Kit Components: 143759A: Microtiter Strips, 1x96 wells *143759B: CD163 Standard, 2x1 vial 143759C: Anti-human CD163 (Biotin), 1x130ul USB Cat No Kit Component 143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution) 143697: Sample Diluent Buffer, 1x30ml 143698: Antibody Diluent Buffer, 1x12ml 143699: ABC Diluent Buffer, 1x12ml 143700: TMB Color Developing Agent, 1x10ml 143701: TMB Stop Solution, 2N H2SO4, 1x10ml Storage and Stability: Store *143759B powder at 4C. Once reconstituted store at 4C for up to 12 hours or at -20C for up to 48 hours. Store other components at 4C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Assay Summary: 1. Add samples and standards and incubate the plate at 37C for 90 minutes. Do not wash. 2. Add biotinylated antibodies and incubate the plate at 37C for 60 minutes. Wash plate 3 times with 0.01M TBS. 3. Add ABC working solution and incubate the plate at 37C for 30 minutes. Wash plate 5 times with 0.01M TBS. 4. Add TMB color developing agent and incubate the plate at 37C in dark for 25-30 minutes. 5. Add TMB stop solution and read.
