Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2 (ENPP2) BioAssay(TM) ELISA Kit (Human)
Artikelnummer:
USB-058940
Hersteller Artikelnummer:
058940
Alternativnummer:
USB-058940-96
Hersteller:
US Biological
Kategorie:
Kits/Assays
For the quantitative determination of human Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 (ENPP2) in serum, plasma, and tissue homogenates. Sensitivity: 31.25ng/ml Detection Range: 25-2000ng/ml Specificity: This assay has high sensitivity and excellent specificity for detection of human ENPP2. No significant cross-reactivity or interference between human ENPP2 and analogues was observed Assay Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ENPP2 antibody. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated ENPP2. The competitive inhibition reaction is launched between with HRP labeled ENPP2 and unlabeled ENPP2 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of ENPP2 in the sample. The color development is stopped and the intensity of the color is measured. Kit Components: Microtiter Strips, 1x96 wells Standard, 6x0.5ml HRP-conjugate, 1x6ml Wash Buffer (20X), 1x15ml Substrate A, 1x7ml Substrate B, 1x7ml Storage and Stability: Store all kit components at 4C. Stable for 6 months after receipt. Once opened, the kit is stable for 1 month providing this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
