Growth Differentiation Factor 1 (GDF1) BioAssay(TM) ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of GDF1 in human plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Detection Range: 0.156-10ng/ml Sensitivity: 0.057ng/ml Intra-Assay CV: <10% Inter-Assay CV: <12% Kit Components: *025475A: Microtiter Strips, 1x96 wells *025475B: Standard, 2x1 vial 025475C: Standard Diluent, 1x20ml *025475D: Detection Reagent A, 1x120ul *025475E: Detection Reagent B, 1x120ul 025475F: Assay Diluent A, 1x12ml 025475G: Assay Diluent B, 1x12ml 025475H: TMB Substrate, 1x9ml *025475J: Positive Control, 1.56ng/ml, 1x1 vial 025475K: Stop Solution, 1x6ml 025475L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (025475K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *025475A, *025475B, *025475D, *025475E and *025475J at -20C. After reconstitution of *025475J, store at 4C and use within 5 days. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
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