5-ROX tetrazine is a derivative of the ROX (Rhodamine X, Rhodamine 101) dye, characterized as a red fluorophore with high brightness and fluorescence quantum yield. This compound contains a tetrazine moiety that can undergo an inverse electron demand Diels-Alder reaction (IEDDA) with trans-cyclooctenes and other strained alkenes, exhibiting exceptional speed and specificity.
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