ELISA Microwell Coating Stabilizer is a ready-to-use 1X solution for stabilizing ELISA plates for storage prior to preforming ELISA. After the addition of blocking agent, wash the contents of ELISA microwells and add a sufficient volume of ELISA Microwel
Rockland Immunochemicals produces a wide variety of buffers and substrates for use in ELISAs. Antigen was diluted in ELISA Microwell Coating Stabilizer (p/n MB-063-0100) added to the microwell plate and incubated overnight at 4C. The plate was then blocked with ELISA Microwell Blocking Buffer with Stabilizer (p/n MB-064-1000) for 2 hours. The primary antibody was diluted in PBS Fish Gel Concentrate (1:10)(p/n MB-066-0100), added to the plate, and allowed to incubate 1 hour at room temperature. HRP conjugated secondary antibody was diluted in HRP Conjugate Stabilizer (p/n MB-060-0100), added to the plate, and allowed to incubate for 30 minutes at room temperature. TMB ELISA Peroxidase Substrate (p/n TMBE-1000) was added to the plate and allowed to incubate for 30 minutes at room temperature. The reaction was then stopped with 1M HCl and read at 450nm.
The Rockland Immunochemical family of ELISA buffers includes coating buffers, blocking buffers, stabilization buffers, and substrates. Rockland ELISA Microwell Coating Stabilizer is specially formulated for stability and sensitivity in ELISA systems.
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