All Lanes:CD86 Antibody at 1/ 1000 dilution.Lane1:Mouse heart.Lane2:Mouse spleen.Lane3:Mouse lung.Lane4:Rat heart.Lane5:Rat liver.(Proteins at 20µg per lane)Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 70kDaObserved band size: 70kDa
All Lanes:CD86 Antibody at 1/ 1000 dilution.Lane1:HeLa.Lane2:Jurkat.Lane3:MCF-7.Lane4:SH-SY5Y.(Proteins at 20µg per lane)Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 70kDaObserved band size: 70kDa
All Lanes:CD86 Antibody at 1/ 1000 dilution.Lane1:Raji cell lysate.Proteins at 20µg per laneSecondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 70kDaObserved band size: 70kDa
Immunofluorescent analysis using the Antibody at 1:50 dilution.4% polyformaldehyde fixed. 0.1% tritonX-100 permeable. DAPI stains the nucleus.
Immunofluorescent analysis of K562 cells, using CD86 Antibody.4% polyformaldehyde fixed. 0.1% tritonX-100 permeable. DAPI stains the nucleus.
Immunohistochemical analysis of paraffin-embedded human tonsil, using CD86 Antibody.Tris-EDTA Buffer (pH9.0).
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