CD80 Antibody [12D9], IgG1, Unconjugated, Mouse, Monoclonal

Artikelnummer: PRS-RF16046
Artikelname: CD80 Antibody [12D9], IgG1, Unconjugated, Mouse, Monoclonal
Artikelnummer: PRS-RF16046
Hersteller Artikelnummer: RF16046
Alternativnummer: PRS-RF16046-0.02,PRS-RF16046-0.1
Hersteller: ProSci
Wirt: Mouse
Kategorie: Antikörper
Applikation: ELISA, ICC, IF, IHC-P, WB
Spezies Reaktivität: Human
Immunogen: CD80 antibody was raised against the extracellular domain of human CD80.
Konjugation: Unconjugated
Alternative Synonym: CD80 Antibody: CD80 molecule, B7, BB1, B7-1, B7.1, LAB7, CD28LG, CD28LG1
Klonalität: Monoclonal
Konzentration: 1 mg/mL
Klon-Bezeichnung: [12D9]
Molekulargewicht: Predicted: 32 kDa Observed: 50 kDa
Isotyp: IgG1
NCBI: 941
UniProt: P33681
Puffer: CD80 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: CD80 antibody can be used for detection of CD80 by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2 - 5 µg/mL. For immunofluorescence start at 5µg/mL.Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples, Immunocytochemistry in human samples, Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
Flow cytometry analysis of CD80 overexpressing HEK293 cells using CD80 antibody and control mouse IgG antibody at 10 &956,g/ml. Blue: Untransfected HEK293 cells. Yellow: CD80 overexpressing HEK293 cells.
Immunocytochemistry of CD80 in transfected HEK293 cells with CD80 antibody at 1 &956,g/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 &956,g/mL.
Immunofluorescence of CD80 in transfected HEK293 cells with CD80 antibody at 2 &956,g/mL. Red: CD80 Antibody [12D9] (RF16046) Blue: DAPI staining
Immunofluorescence of CD80 in human stomach carcinoma tissue with CD80 antibody at 20 &956,g/mL. Red: CD80 Antibody [12D9] (RF16046) Blue: DAPI staining
Immunofluorescence of CD80 in human tonsil tissue with CD80 antibody at 2 &956,g/mL. Red: CD80 Antibody [12D9] (RF16046) Blue: DAPI staining
Immunohistochemistry of CD80 in human stomach carcinoma tissue with CD80 antibody at 5 &956,g/mL.
Immunohistochemistry of CD80 in human tonsil tissue with CD80 antibody at 5 &956,g/mL.
Western blot analysis of CD80 in overexpressing HEK293 cells CD80 antibody at 0.25 and 0.5 &956,g/ml