PEX3 (CT) Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
PRS-9665
- Bilder (7)
| Artikelname: | PEX3 (CT) Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | PRS-9665 |
| Hersteller Artikelnummer: | 9665 |
| Alternativnummer: | PRS-9665-0.02,PRS-9665-0.1 |
| Hersteller: | ProSci |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | Anti-PEX3 antibody (9665) was raised against a peptide corresponding to 12 amino acids near the carboxyl terminal of human PEX3. The immunogen is located within last 50 amino acids of PEX3. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | PEX3 Antibody: Peroxisomal biogenesis factor 3, Peroxin-3, Peroxisomal assembly protein PEX3 |
| Application Verdünnung: | Optimal dilutions for each application to be determined by the researcher. |
| Anwendungsbeschreibung: | WB: 2 µg/mL, IF: 20 µg/mL. Antibody validated: Western Blot in human, mouse and rat samples, Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested. |
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Figure 4 Immunofluorescence Validation of PEX3 in Human HeLa CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling PEX3 with 9665 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). |
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Figure 5 Immunofluorescence Validation of PEX3 in Human SpleenImmunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling PEX3 with 9665 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). |
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Figure 6 Immunofluorescence Validation of PEX3 in Mouse ThymusImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus labeling PEX3 with 9665 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). |
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Figure 7 Immunofluorescence Validation of PEX3 in Rat testisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX3 with 9665 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). |
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Figure 1 WB Validation in Human Cell Lines Loading: 15 &956,g of lysate Antibodies: PEX3 9665, 2 &956,g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution. |
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Figure 2 Western Blot Validation in Mouse Tissues Loading: 15 &956,g of lysates per lane.Antibodies: PEX3 9665, 2 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. |
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Figure 3 Western Blot Validation in Rat TissuesLoading: 15 &956,g of lysates per lane.Antibodies: PEX3 9665, 2 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. |
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