NCOA4 (IN) Antibody, Unconjugated, Rabbit, Polyclonal

Artikelname:	NCOA4 (IN) Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	PRS-9663
Hersteller Artikelnummer:	9663
Alternativnummer:	PRS-9663-0.02,PRS-9663-0.1
Hersteller:	ProSci
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ELISA, IF, IHC, WB
Spezies Reaktivität:	Human, Mouse, Rat
Immunogen:	Anti-NCOA4 antibody (9663) was raised against a peptide corresponding to 13 amino acids near the center of human NCOA4. The immunogen is located within 420-470 amino acids of NCOA4.
Konjugation:	Unconjugated
Alternative Synonym:	NCOA4 Antibody: Nuclear receptor coactivator 4, NCoA-4, Androgen receptor coactivator 70 kDa protein, 70 kDa AR-activator, 70 kDa androgen receptor coactivator, Androgen receptor-associated protein of 70 kDa, Ret-activating protein ELE1, ARA70, ELE1, RFG

Klonalität:	Polyclonal
Konzentration:	1 mg/mL
Molekulargewicht:	Predicted: 70-74 kDObserved: 70-74 kD
NCBI:	8031
UniProt:	Q13772
Puffer:	NCOA4 Antibody is supplied in PBS containing 0.02% sodium azide.
Formulierung:	Liquid

Application Verdünnung:	Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:	WB: 0.5-1 µg/mL, IHC: 1 µg/mL, IF: 20 µg/mL. Antibody validated: Western Blot in human, mouse and rat samples, Immunofluorescence in human samples and Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.

	Figure 4: Immunofluorescence Validation of NCOA4 in Daudi CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling NCOA4 with 9663 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Figure 5: Immunohistochemistry Validation of NCOA4 in Human lungImmunohistochemical analysis of paraffin-embedded human mouse tissue using anti-NCOA4 antibody (9663) at 1 &956,g/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 6: Immunohistochemistry Validation of NCOA4 in Mouse lungImmunohistochemical analysis of paraffin-embedded mouse tissue using anti-NCOA4 antibody (9663) at 1 &956,g/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 7: Immunohistochemistry Validation of NCOA4 in Rat lungImmunohistochemical analysis of paraffin-embedded rat tissue using anti-NCOA4 antibody (9663) at 1 &956,g/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 1: Western Blot Validation in Human Cell LinesLoading: 15 &956,g of lysates per lane. Antibodies: NCOA4 9663, 0.5 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
	Figure 2: Western Blot Validation in Mouse Tissues Loading: 15 &956,g of lysates per lane. Antibodies: NCOA4 9663, 1 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
	Figure 3: Western Blot Validation in Rat TissuesLoading: 15 &956,g of lysates per lane.Antibodies: NCOA4 9663, 1 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.