TET1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelname:	TET1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	PRS-7733
Hersteller Artikelnummer:	7733
Alternativnummer:	PRS-7733-0.02,PRS-7733-0.1
Hersteller:	ProSci
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ELISA, IF, IHC-P, WB
Spezies Reaktivität:	Human, Mouse, Rat
Immunogen:	Anti-TET1 antibody (7733) was raised against an 18 amino acid peptide near the carboxy terminus of human TET1. The immunogen is located within amino acids 2030 - 2080 of TET1.
Konjugation:	Unconjugated
Alternative Synonym:	TET1 Antibody: LCX, CXXC6, bA119F7.1, KIAA1676, LCX, Methylcytosine dioxygenase TET1, CXXC-type zinc finger protein 6

Klonalität:	Polyclonal
Konzentration:	1 mg/mL
Molekulargewicht:	Predicted: 235 kDa Observed: 235 kDa
NCBI:	80312
UniProt:	Q8NFU7
Puffer:	TET1 Antibody is supplied in PBS containing 0.02% sodium azide.
Formulierung:	Liquid

Application Verdünnung:	Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:	WB: 1 µg/mL, IHC: 1 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot and Immunohistochemistry in human, mouse and rat samples, Immunocytochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

	Figure 4 Immunofluorescence Validation of TET1 in HeLa CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Hela cells labeling TET1 with 7733 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).
	Figure 5Immunofluorescence Validation of TET1 in Mouse Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling TET1 with 7733 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI antibody (blue).
	Figure 6 Immunohistochemistry Validation of TET1 in Human Testis Tissue Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-TET1 antibody (7733) at 1 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 7 Immunohistochemistry Validation of TET1 in Mouse Kidney Tissue Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TET1 antibody (7733) at 1 &956,g /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 8 Immunohistochemistry Validation of TET1 in Rat Liver Tissue Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-TET1 antibody (7733) at 1 &956,g /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 1 WB Validation of TET1 in Human Cell Lines Loading: 15 &956,g of lysate Antibodies: TET1 7733, 1 &956,g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 2 WB Validation of TET1 in Mouse Tissues Loading: 15 &956,g of lysate Antibodies: TET1 7733 , 1 &956, g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 3 WB Validation of TET1 in Rat Tissues Loading: 15 &956,g of lysate Antibodies: TET1 7733, 1 &956,g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.