JMJD3 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
PRS-6657
- Bilder (8)
| Artikelname: | JMJD3 Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | PRS-6657 |
| Hersteller Artikelnummer: | 6657 |
| Alternativnummer: | PRS-6657-0.02,PRS-6657-0.1 |
| Hersteller: | ProSci |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, ICC, IF, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | Anti-JMJD3 antibody (6657) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human JMJD3. The immunogen is located within amino acids 1370-1420 of JMJD3. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | JMJD3 Antibody: JMJD3, JMJD3, KIAA0346, Lysine-specific demethylase 6B, JmjC domain-containing protein 3 |
| Application Verdünnung: | Optimal dilutions for each application to be determined by the researcher. |
| Anwendungsbeschreibung: | WB: 0.25-4 µg/mL, ICC: 2.5 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunocytochemistry in human samples, Immunofluorescence in human samples. All other applications and species not yet tested. |
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Figure 3 Western Blot Validation in HeLa Cell LysateLoading: 15 &956,g of lysates per lane.Antibodies: JMJD3, 6657 (0.25 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
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Figure 7 Immunocytochemistry Validation of JMJD3 in K562 CellsImmunocytochemical analysis of K562 cells using anti-JMJD3 antibody (6657) at 2.5 &956,g/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. |
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Figure 8 Immunofluorescence Validation of JMJD3 in K562 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling JMJD3 with 6657 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). |
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Figure 1 Western Blot Validation in Human, Mouse and Rat Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: JMJD3, 6657, (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
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Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines Loading: 15 &956,g of lysates per lane.Antibodies: JMJD3 6657 (1 &956,g/mL), JMJD3 14-618, (2 &956,g/mL) and beta-actin 3779 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
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Figure 4 Western Blot Validation in K562 Cell Lysate Loading: 15 &956,g of lysates per lane.Antibodies: : JMJD3, 6657 (0.5 &956,g/mL) , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: in the absence and Lane 2: in the presence of blocking peptide |
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Figure 5 Western Blot Validation in Mouse A-20 Cell Lysate Loading: 15 &956,g of lysates per lane.Antibodies: : JMJD3, 6657 (0.5 &956,g/mL) , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: in the absence and Lane 2: in the presence of blocking peptide |
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Figure 6 Western Blot Validation in HepG2 Cell Lysate Loading: 15 &956,g of lysates per lane.Antibodies: : JMJD3, 6657, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.25 &956,g/mLLane 2: 0.5 &956,g/mL Lane 3: 0.25 &956,g/mL with blocking peptide |
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