This UCHL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-46 amino acids from the N-terminal region of human UCHL1.
Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:
For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
Western Blot at 1:1000 dilution Lane 1: DU145 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of UCHL1 using rabbit polyclonal UCHL1-V31 using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UCHL1 gene (Lane 2).
Western blot analysis of UCHL1-V31 in CEM(lane 1), Jurkat(lane 2), Y79(lane 3) cell line and mouse brain tissue(lane 4) lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with UCHL1 (Park5) antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Confocal immunofluorescent analysis of UCHL1 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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