This CD130 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 871-899 amino acids from the C-terminal region of human CD130.
Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:
For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:25
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: MDA-MB-231 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: SW480 whole cell lysate Lane 5: A549 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: HT-1080 whole cell lysate Lysates/proteins at 20 ug per lane.
Antibody staining CD130 in Human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: MDA-MB-231 whole cell lysate Lane 3: SW480 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + SW480 whole cell lysates Lysates/proteins at 20 ug per lane.
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
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