This SLC16A11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-76 amino acids from the N-terminal region of human SLC16A11.
Konjugation:
Unconjugated
Alternative Synonym:
Monocarboxylate transporter 11, MCT 11, Solute carrier family 16 member 11, SLC16A11, MCT11
Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:
For FACS starting dilution is: 1:25For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:500
Overlay histogram showing HT-29 cells stained with Antibody (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10 6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Overlay histogram showing HT-29 cells stained with Antibody (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10 6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Antibody staining SLC16A11 in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:1000 dilution + mouse liver lysates Lysates/proteins at 20 ug per lane.
Western Blot at 1:500 dilution + A549 whole cell lysates Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + mouse brain lysates Lysates/proteins at 20 ug per lane.
SLC16A11 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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