This CD11b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-282 amino acids from the N-terminal region of human CD11b.
Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:
For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:500For FACS starting dilution is: 1:10~50
Antibody staining D11b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Antibody staining D11b in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Antibody staining D11b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution + human spleen lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:500 dilution + human spleen lysate Lysates/proteins at 20 ug per lane.
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in A549,K562 cell line lysates (35ug/lane).
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