NALP3 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelname:	NALP3 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	PRS-5447
Hersteller Artikelnummer:	5447
Alternativnummer:	PRS-5447-0.02,PRS-5447-0.1
Hersteller:	ProSci
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ELISA, IF, IHC, WB
Spezies Reaktivität:	Human, Mouse, Rat
Immunogen:	Anti-NALP3 antibody (5447) was raised against a 16 amino acid synthetic peptide from near the amino terminus of human NALP3. The immunogen is located within amino acids 120 - 170 of NALP3.
Konjugation:	Unconjugated
Alternative Synonym:	NALP3 Antibody: AII, AVP, FCU, MWS, FCAS, CIAS1, NALP3, C1orf7, CLR1.1, PYPAF1, AGTAVPRL, NACHT, LRR and PYD domains-containing protein 3, Cold autoinflammatory syndrome 1 protein

Klonalität:	Polyclonal
Konzentration:	1 mg/mL
Molekulargewicht:	Predicted: 118 kDa Observed: 118 kDa
NCBI:	114548
UniProt:	Q96P20
Puffer:	NALP3 Antibody is supplied in PBS containing 0.02% sodium azide.
Formulierung:	Liquid

Application Verdünnung:	Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:	WB: 0.5 µg/mL, IF: 20 µg/mL. Antibody validated: Western Blot in human, mouse and rat samples, Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.

	Figure 4 Immunofluorescence Validation of NALP3 in Human K562 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed K562 labeling NALP3 with 5447 at 5 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
	Figure 5 Immunofluorescence Validation of NALP3 in Human Molt4 Cells Immunofluorescent analysis of 4% paraformaldehyde-fixed Molt4 labeling NALP3 with 5447 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Figure 6 Immunofluorescence Validation of NALP3 in Mouse ThymusImmunohistochemical analysis of paraffin-embedded mouse thymus tissue using anti-NALP3 antibody (5447) at 1 &956,g/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 7 Immunohistochemistry Validation of NALP3 in Rat LiverImmunohistochemical analysis of paraffin-embedded rat liver tissue using anti-NALP3 antibody (5447) at 1 &956,g/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 1 WB Validation in Human, Mouse and Rat Cell LinesLoading: 15 &956,g of lysate. Antibodies: NALP3 5447, 0.5 &956,g/mL , 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
	Figure 2 Western Blot Validation in Mouse Tissues Loading: 15 &956,g of lysates per lane. Antibodies: NALP3 5447, 0.5 &956,g/mL, 1h incubation at RT in 8% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
	Figure 3 Western Blot Validation in Rat TissuesLoading: 15 &956,g of lysates per lane. Antibodies: NALP3 5447, 0.5 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.