MPYS Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
PRS-5269
- Bilder (6)
| Artikelname: | MPYS Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | PRS-5269 |
| Hersteller Artikelnummer: | 5269 |
| Alternativnummer: | PRS-5269-0.02,PRS-5269-0.1 |
| Hersteller: | ProSci |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, ICC, IF, WB |
| Spezies Reaktivität: | Human, Mouse |
| Immunogen: | Anti-MPYS antibody (5269) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human MPYS. The immunogen is located within amino acids 260-310 of MPYS. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | MPYS Antibody: ERIS, MITA, MPYS, NET23, STING, ERIS, Stimulator of interferon genes protein, Endoplasmic reticulum interferon stimulator, hSTING |
| Application Verdünnung: | Optimal dilutions for each application to be determined by the researcher. |
| Anwendungsbeschreibung: | WB: 1-2 µg/mL, ICC: 5 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human and mouse samples, Immunocytochemistry and Immunofluorescence in mouse samples. All other applications and species not yet tested. |
|
|
Figure 5 Immunocytochemistry Validation of MPYS in Mouse A20 CellsImmunocytochemical analysis of A20 cells using anti-MPYS antibody (5269) at 5 &956,g/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. |
|
|
Figure 6 Immunofluorescence Validation of MPYS in Moue A20 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed A20 Cells labeling MPYS with 5269 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). |
|
|
Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: MPYS 5269 (1 &956,g/mL), MPYS 64-236 (2 &956,g/mL) and beta-actin 3779 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
|
|
Figure 2 Western Blot Validation in Human Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: MPYS 5269 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
|
|
Figure 3 Western Blot Validation in Human THP-1 Cell LysateLoading: 15 &956,g of lysates per lane.Antibodies: MPYS 5269 (Lane 1: 1 &956,g/mL and Lane 2: 2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
|
|
Figure 4 Western Blot Validation in Mouse A-20 Cell LysateLoading: 15 &956,g of lysates per lane.Antibodies: MPYS 5269 (Lane 1: 1 &956,g/mL and Lane 2: 2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
Produktgarantie und fachkundiger Support
