RSPO1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelname:	RSPO1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	PRS-5171
Hersteller Artikelnummer:	5171
Alternativnummer:	PRS-5171-0.02,PRS-5171-0.1
Hersteller:	ProSci
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ELISA, IF, IHC-P, WB
Spezies Reaktivität:	Human, Mouse
Immunogen:	Anti-RSPO1 antibody (5171) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human RSPO1. The immunogen is located within the first 50 amino acids of RSPO1.
Konjugation:	Unconjugated
Alternative Synonym:	RSPO1 Antibody: RSPO, CRISTIN3, R-spondin-1, Roof plate-specific spondin-1, hRspo1

Klonalität:	Polyclonal
Konzentration:	1 mg/mL
Molekulargewicht:	Predicted: 29kDObserved: 36 kD (Post-modification: 1 N-linked glycosylation)
NCBI:	284654
UniProt:	Q2MKA7
Puffer:	RSPO1 Antibody is supplied in PBS containing 0.02% sodium azide.
Formulierung:	Liquid

Application Verdünnung:	Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:	WB: 0.5-2 µg/mL, IHC: 2.5-5 µg/mL, IF: 5 µg/mL.Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry in human samples, Immunofluorescence in human samples. All other applications and species not yet tested.

	Figure 4 Western Blot Validation in Human HeartLoading: 15 &956,g of lysates per lane.Antibodies: RSPO1, 5171 (A: 1 &956,g/mL, B: 2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 5 Immunofluorescence Validation of RSPO1 in Human Spleen Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling RSPO1 with 5171 at 5 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) with Phylloidin staining (red) and DAPI staining (blue).
	Figure 8 Immunohistochemistry Validation of RSPO1 in Human Spleen Tissue Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-RSPO1 antibody (5171) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 6 Immunohistochemistry Validation of RSPO1 in Human Heart Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-RSPO1 antibody (5171) at 2.5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 7 Immunohistochemistry Validation of RSPO1 in Human Spleen Tissue Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-RSPO1 antibody (5171) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 1 Western Blot Validation in Human and Mouse Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: RSPO1, 5171 (2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 2 Western Blot Validation with Recombinant ProteinLoading: 30 ng of human RSPO1 recombinant protein per lane.Antibodies: RSPO1, 5171 (Lane 1: 0.5 &956,g/mL, Lane 2: 1 &956,g/mL, Lane 3: 2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 3 Western Blot Validation in Human Spleen Loading: 15 &956,g of lysates per lane.Antibodies: RSPO1, 5171 (Lane 1: 0.5 &956,g/mL, Lane 2: 1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.