TOM70 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelname:	TOM70 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	PRS-4959
Hersteller Artikelnummer:	4959
Alternativnummer:	PRS-4959-0.02,PRS-4959-0.1
Hersteller:	ProSci
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ELISA, IF, IHC-P, WB
Spezies Reaktivität:	Human, Mouse, Rat
Immunogen:	Anti-TOM70 antibody (4959) was raised against a peptide corresponding to 15 amino acids near the center of human TOM70. The immunogen is located within amino acids 220-270 of TOM70.
Konjugation:	Unconjugated
Alternative Synonym:	TOM70 Antibody: KIAA0719, TOM70, Mitochondrial import receptor subunit TOM70, Mitochondrial precursor proteins import receptor

Klonalität:	Polyclonal
Konzentration:	1 mg/mL
Molekulargewicht:	Predicted: 68kDObserved: 72 kD
NCBI:	9868
UniProt:	O94826
Puffer:	TOM70 Antibody is supplied in PBS containing 0.02% sodium azide.
Formulierung:	Liquid

Application Verdünnung:	Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:	WB: 1-2 µg/mL, IHC: 2.5 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry and Immunofluorescence in mouse samples. All other applications and species not yet tested.

	Figure 7 Immunofluorescence Validation of TOM70 in Mouse Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling TOM70 with 4959 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
	Figure 6 Immunohistochemistry Validation of TOM70 in Mouse Brain Tissue Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TOM70 antibody (4959) at 2.5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: TOM70 4949 (2 &956,g/mL), TOM70 4959 (2 &956,g/mL) and beta-actin (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 2 Western Blot Validation in Human and Mouse Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: TOM70 4959 (2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 3 Western Blot Validation in Human Brain Loading: 15 &956,g of lysates per lane.Antibodies: TOM70 4959 (Lane 1: 1 &956,g/mL and Lane 2: 2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 4 Western Blot Validation in Mouse BrainLoading: 15 &956,g of lysates per lane.Antibodies: TOM70 4959 (Lane 1: 1 &956,g/mL and Lane 2: 2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 5 Western Blot Validation in Rat Brain Loading: 15 µg of lysates per lane.Antibodies: TOM70 4959 (A: 1 &956,g/mL and B: 2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.