TOM70 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
PRS-4949
- Bilder (6)
| Artikelname: | TOM70 Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | PRS-4949 |
| Hersteller Artikelnummer: | 4949 |
| Alternativnummer: | PRS-4949-0.02,PRS-4949-0.1 |
| Hersteller: | ProSci |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | Anti-TOM70 antibody (4949) was raised against a peptide corresponding to 17 amino acids near the amino terminus of human TOM70. The immunogen is located within amino acids 50-100 of TOM70. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | TOM70 Antibody: KIAA0719, TOM70, Mitochondrial import receptor subunit TOM70, Mitochondrial precursor proteins import receptor |
| Application Verdünnung: | Optimal dilutions for each application to be determined by the researcher. |
| Anwendungsbeschreibung: | WB: 1-4 µg/mL, IHC: 2.5 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry and Immunofluorescence in mouse samples. All other applications and species not yet tested. |
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Figure 6 Immunofluorescence Validation of TOM70 in Mouse Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling TOM70 with 4949 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). |
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Figure 5 Immunohistochemistry Validation of TOM70 in Mouse Brain Tissue Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TOM70 antibody (4949) at 2.5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 &730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. |
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Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: TOM70 4949 (2 &956,g/mL), TOM70 4959 (2 &956,g/mL) and beta-actin (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
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Figure 2 Western Blot Validation in Human and Mouse Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: TOM70 4949 (2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
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Figure 3 Western Blot Validation in Mouse Brain Loading: 15 &956,g of lysates per lane.Antibodies: TOM70 4949 (Lane 1: 2 &956,g/mL and Lane 2: 4 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
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Figure 4 Western Blot Validation in Rat Brain Loading: 15 µg of lysates per lane.Antibodies: TOM70 4949 (Lane 1: 2 &956,g/mL and Lane 2: 4 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
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