Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Formulierung:
Liquid
Anwendungsbeschreibung:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40kDa band observed in Human Heart lysates. The observed molecular weight corresponds to glycosylation (calculated MW of 33.3kDa according to NP_054862.1). An additional band was also consistently observed at 55kDa, and was successfully blocked by incubation with the immunizing peptide. Preliminary testing also showed the 37+55kDa bands in lysates of cell lines, A549, Daudi, HeLa, HepG2 and Jurkat Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Placenta shows membranous staining of cytotrophoblasts. Recommended concentration: 2-4ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
46-156 (0.3ug/ml) staining of Human Heart (A) lysate + Blocking peptide (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-156 (2ug/ml) staining of paraffin embedded Human Placenta. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
46-156 Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.
46-156 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
46-156 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
46-156 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f
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