Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Formulierung:
Liquid
Anwendungsbeschreibung:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 110kDa band observed in Rat Pancreas lysates calculated MW of 116kDa according to NP_858058.2). An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.05-0.2ug/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Mouse Brain for this particular batch.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 5ug/ml.Immunofluorescence: Strong expression of the protein seen in the nucleus of HeLa, U2OS and Glioblastoma U251 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.
45-995 (0.05ug/ml) staining of Rat Pancreas lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-995 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and membrane/cytoplasmic staining. Actin f
45-995 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f
45-995 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stai
45-995 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-995 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained wit
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