PD-1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelname:	PD-1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	PRS-4065
Hersteller Artikelnummer:	4065
Alternativnummer:	PRS-4065-0.02,PRS-4065-0.1
Hersteller:	ProSci
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ELISA, IF, IHC-P, WB
Spezies Reaktivität:	Human, Mouse, Rat
Immunogen:	Anti-PD-1 antibody (4065) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human PD-1. The immunogen is located within amino acids 210-260 of PD-1.
Konjugation:	Unconjugated
Alternative Synonym:	PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, PD1, Programmed cell death protein 1, Protein PD-1, PDCD1, PDCD-1

Klonalität:	Polyclonal
Konzentration:	1 mg/mL
Molekulargewicht:	Human PD-1 has 1 isoform (288aa, 32kD). Mouse PD-1 has 1 isoform 288aa, 32kD) and Rat PD-1 also has one isoform (287aa, 32kD). 4065 can detect human, mouse and rat.
NCBI:	5133
UniProt:	Q15116
Puffer:	PD-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Formulierung:	Liquid

Application Verdünnung:	Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:	WB: 1-4 µg/mL, IHC: 2.5-5 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in human samples, Immunofluorescence in human samples. All other applications and species not yet tested.

	Figure 4 Western Blot Validation in THP-1 Cell Lysate in the (A) absence and (B) presence of blocking peptideLoading: 15 &956,g of lysates per lane.Antibodies: PD-1, 4065 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 5 Western Blot Validation in Rat Thymus Cell LysateLoading: 15 &956,g of lysates per lane.Antibodies: PD-1 4065 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 6 Immunohistochemistry Validation of PD-1 in Human Tonsil Tissue Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PD-1 antibody (4065) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 8 Immunohistochemistry Validation of PD-1 in Human Brain Tissue Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-PD-1 antibody (4065) at 2.5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 6 Immunohistochemistry Validation of PD-1 in Human Tonsil Tissue Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PD-1 antibody (4065) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 1 KO Validation in HeLa Cells Loading: 10 &956,g of HeLa WT cell lysates or PD-1 KO cell lysates. Antibodies: PD-1, 4065 (4 &956,g/mL) and beta-actin 3779 (1 &956,g/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 2 KD Validation in HeLa Cells Loading: 10 &956,g of HeLa WT cell lysates or PD-1 KD cell lysates. Antibodies: PD-1, 4065 (4 &956,g/mL) and beta-actin 3779 (1 &956,g/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 3 Western Blot Validation in Human and Mouse Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: PD-1 4065 (4 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.