PAK2 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
PRS-3887
- Bilder (6)
| Artikelname: | PAK2 Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | PRS-3887 |
| Hersteller Artikelnummer: | 3887 |
| Alternativnummer: | PRS-3887-0.02,PRS-3887-0.1 |
| Hersteller: | ProSci |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | Anti-PAK2 antibody (3887) was raised against a peptide corresponding to 14 amino acids near the amino terminus of human PAK2. The immunogen is located within amino acids 20-70 of PAK2. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | PAK2 Antibody: PAK65, PAKgammaGamma-PAK, PAK-2 |
| Application Verdünnung: | Optimal dilutions for each application to be determined by the researcher. |
| Anwendungsbeschreibung: | WB: 0.5-2 µg/mL, IHC: 10 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry and Immunofluorescence in mouse samples. All other applications and species not yet tested. |
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Figure 1 Western Blot Validation in Jurkat LysateLoading: 15 &956,g of lysates per lane.Antibodies: PAK2 3887 (A: 0.5 &956,g/mL, B: 1 &956,g/mL and C: 2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
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Figure 6 Immunofluorescence Validation of PAK2 in Mouse Spleen TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse spleen tissue labeling PAK2 with 3887 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). |
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Figure 5 Immunohistochemistry Validation of PAK2 in Mouse Spleen Tissue Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PAK2 antibody (3887) at 10 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. |
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Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: PAK2 3885 (1 &956,g/mL), PAK2 3887 (1 &956,g/mL) and beta-actin (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
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Figure 3 Western Blot Validation in Human Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: PAK2 3887 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
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Figure 4 Western Blot Validation in Mouse TissuesLoading: 15 &956,g of lysates per lane.Antibodies: PAK2 3887 (1 &956,g/mL per lane), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
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