Produced from sera of rabbits immunized with highly pure recombinant Human Maspin. Human Maspin specific antibody was purified by affinity chromatography employing an immobilized Human Maspin matrix.
ELISA:In a sandwich ELISA (using 100 uL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Maspin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMaspin. Western Blot:To detect hMaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hMaspin by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProScis Biotinylated Anti-Human Maspin (38-278) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMaspin.
This antibody stained formalin-fixed paraffin-embedded sections of human normal breast and human breast lobular carcinoma in situ. The recommended concentration is 7.8 ng/mL- 15.625 ng/mL with an overnight incubation at 4&730,C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed paraffin-embedded sections of human normal breast and human breast lobular carcinoma in situ. The recommended concentration is 7.8 ng/mL- 15.625 ng/mL with an overnight incubation at 4&730,C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed paraffin-embedded sections of human normal breast and human breast lobular carcinoma in situ. The recommended concentration is 7.8 ng/mL- 15.625 ng/mL with an overnight incubation at 4&730,C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary.
To detect hMaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hMaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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