IL-23 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelname:	IL-23 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	PRS-3795
Hersteller Artikelnummer:	3795
Alternativnummer:	PRS-3795-0.02,PRS-3795-0.1
Hersteller:	ProSci
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ELISA, IF, IHC-P, WB
Spezies Reaktivität:	Human, Mouse
Immunogen:	Anti-IL-23 antibody (3795) was raised against a peptide corresponding to 17 amino acids near the amino terminus of human IL-23. The immunogen is located within amino acids 40 - 90 of IL-23.
Konjugation:	Unconjugated
Alternative Synonym:	IL-23 Antibody: P19, SGRF, IL-23, IL-23A, IL23P19, UNQ2498/PRO5798, Interleukin-23 subunit alpha, Interleukin-23 subunit p19, IL-23 subunit alpha

Klonalität:	Polyclonal
Konzentration:	1 mg/mL
Molekulargewicht:	Predicted: 21kDObserved: 22 kD
NCBI:	51561
UniProt:	Q9NPF7
Puffer:	IL-23 Antibody is supplied in PBS containing 0.02% sodium azide.
Formulierung:	Liquid

Application Verdünnung:	Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:	WB: 0.25-2 µg/mL, IF: 20 µg/mL, IHC: 2 µg/mL.Antibody validated: Western Blot in human and mouse samples, Immunofluorescence and Immunohistochemistry in mouse samples. All other applications and species not yet tested.

	Figure 4 Immunofluorescence Validation of IL-23 in Mouse Pancreas TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse pancreas tissue labeling IL-23 with 3795 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
	Figure 5 Immunohistochemistry Validation of IL-23 in Mouse Pancreas Tissue Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-IL-23 antibody (3795) at 2 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730, C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 6 Induced Expression Validation of IL-23 in Mouse Keratinocytes of Epidermis (Yoon et al., 2016) The expression of IL-23 protein induced by tape stripping was detected by immunohistochemical analysis of mouses skin with anti-IL-23 antibodies. Skin sections from the mouse exhibited epidermis staining positive for IL-23 expression 6 hr after tape stripping. Staining with an isotype-matched irrelevant antibody (Ctrl IgG) was used as a negative control.
	Figure 1 Western Blot Validation in Mouse Pancreas Tissue LysateLoading: 15 &956,g of lysates per lane.Antibodies: IL-23 3795 (A: 1 &956,g/mL, B: 2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 2 Western Blot Validation with Recombinant ProteinLoading: 30 ng of human IL-23 recombinant protein per lane.Antibodies: IL-23 3795 (1: 0.25 &956,g/mL, 2: 0.5 &956,g/mL, 3: 1 &956,g/mL and 4: 2&956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 3 Western Blot Validation in Mouse Tissue LysateLoading: 15 &956,g of lysates per lane.Antibodies: IL-23 3795 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.