IRAK-4 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelname:	IRAK-4 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	PRS-3125
Hersteller Artikelnummer:	3125
Alternativnummer:	PRS-3125-0.02,PRS-3125-0.1
Hersteller:	ProSci
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ELISA, ICC, IF, WB
Spezies Reaktivität:	Human
Immunogen:	Anti-IRAK4 antibody (3125) was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human IRAK4. The immunogen is located within the last 50 amino acids of IRAK4.
Konjugation:	Unconjugated
Alternative Synonym:	IRAK-4 Antibody: IPD1, REN64, IRAK-4, NY-REN-64, Interleukin-1 receptor-associated kinase 4, Renal carcinoma antigen NY-REN-64

Klonalität:	Polyclonal
Konzentration:	1 mg/mL
Molekulargewicht:	Predicted: 52kDObserved: 55 kD
NCBI:	51135
UniProt:	Q9NWZ3
Puffer:	IRAK-4 Antibody is supplied in PBS containing 0.02% sodium azide.
Formulierung:	Liquid

Application Verdünnung:	Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:	WB: 1-4 µg/mL, ICC: 10 µg/mL, IF: 10 µg/mL.Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

	Figure 4 Immunocytochemistry Validation of IRAK4 in K562 CellsImmunocytochemical analysis of K562 cells using anti-IRAK4 antibody (3125) at 10 &956,g/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730, C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 5 Immunofluorescence Validation of IRAK4 in K562 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed K562 Cells labeling IRAK4 with 3125 at 10 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
	Figure 3 Western Blot Validation in Human HeLa Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: IRAK4 3125, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 1 &956,g/mLLane 2: 2 &956,g/mLLane 3: 4 &956,g/mL
	Figure 1 Western Blot Validation in Human Cell LinesLoading: 15 &956,g/ of lysates per lane.Antibodies: IRAK4 3125 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: IRAK4-3125 (1 &956,g/mL), IRAK4-24-025 (1 &956,g/mL), beta-actin (1.5 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 6 KO and KD Validation of IRAK4 in Mouse Bone Marrow-derived Macrophages (BMDM) (Koziczak-Holbro et al., 2008) Western blot analysis with anti-IRAK4 antibodies was performed for IRAK4 in BMDM isolated from the mice. IRAK4 expression was not observed in the IRAK-4-/- cells and also reduced in IRAK4 mutant (IRAK-4 KD) when compared with WT.
	Figure 7 Regulated Expression Validation of IRAK4 in Mouse RAW 264 Cells (Hatao et al., 2004) RAW 264 cells were stimulated with (a) CSK4 and (b) CpG-DNA in the absence or presence of proteasome inhibitors (MG132 and Lactactstin). When detected with anti-IRAK4 antibodies (C12 from ProSci, Inc.), IRAK4 expression was found to be reduced without the inhibitors. The smaller protein band, a cleavage product of IRAK4, was present in the absence of both inhibitors.
	Figure 8 KD Validation of IRAK4 in HEK293T Cells (Heinz et al., 2012) Western blot analysis with anti-IRAK4 antibodies was performed for IRAK4 in HEK293T cells. IRAK4 expression was not observed in IRAK4 knockdown cells.