WNT9A Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-23-293
Artikelname: WNT9A Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-23-293
Hersteller Artikelnummer: 23-293
Alternativnummer: PRS-23-293-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 206-365 of human WNT9A (NP_003386.1).
Konjugation: Unconjugated
Alternative Synonym: Protein Wnt-9a, Protein Wnt-14, WNT9A, WNT14
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 30kDa
NCBI: 7483
UniProt: O14904
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of mouse lung, using WNT9A antibody (23-293) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using WNT9A antibody (23-293) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using WNT9A antibody (23-293) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using WNT9A antibody (23-293) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using WNT9A antibody (23-293) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using WNT9A antibody (23-293) at dilution of 1:100. Blue: DAPI for nuclear staining.