RIPK1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-22-922
Artikelname: RIPK1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-22-922
Hersteller Artikelnummer: 22-922
Alternativnummer: PRS-22-922-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, IP, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 170-440 of human RIPK1 (NP_003795.2).
Konjugation: Unconjugated
Alternative Synonym: RIPK1 Antibody: RIP, RIP1, RIP, Cell death protein RIP, RIP-1
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 76kDa
NCBI: 8737
UniProt: Q13546
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using RIPK1 antibody (22-922) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using RIPK1 antibody (22-922) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human tonsil using RIPK1 antibody (22-922) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using RIPK1 antibody (22-922) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using RIPK1 antibody (22-922) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using RIPK1 antibody (22-922) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using RIPK1 antibody (22-922) at dilution of 1:100. Blue: DAPI for nuclear staining.